Just a quick note following that cell count. I made the samples up in 100ml batches. I checked back just now - going to clean up and wash out the beakers. the water batch had settled out and there was no action at all. Just plain old water with a sludge of yeast in the bottom. The sample in the wort was fizzing away with a good head of froth on top. This is 3 1/2 hours after I started to re-hydrate the yeast. So perhaps re-hydrate in water but use it fairly smartish. Or maybe have some wort ready, re-hydrate for a few minutes in water and then add to a starter say, 1l of wort within 15 minutes or so. Then pitch at your leisure.
Incidentally, for the cell counts I used Methylene Blue as the dye and the top image was water and the bottom was wort (the MB colours the wort a little), otherwise the settings were exactly the same. The images were taken at 400x (2700 x 1800 px), but then reduced for uploading so they don't take up too much bandwidth.
Incidentally, for the cell counts I used Methylene Blue as the dye and the top image was water and the bottom was wort (the MB colours the wort a little), otherwise the settings were exactly the same. The images were taken at 400x (2700 x 1800 px), but then reduced for uploading so they don't take up too much bandwidth.