Smiddylad said:
It depends how you harvest it. Top cropping is the best way as you get the most viable yeast at high krausen. If you harvest your yeast this way there is no real limit to how many times you can repitch it.
Neil
This isnt always true....though you will get very active yeast, taking it from the top all the time will eventually change some attributes of the original yeasts...
Especially alcohol tolerance for example....if you took the yeast off the first krausen EVERY time then over a short period those yeast cells will eventually loose their original tolerance to high alcohol concentrations...
Natural selection or evolution applies to everything that reproduces..whether its sexual or asexual....any organisms attributes are moulded and shaped by external pressures of the environments they live in...
High alcohol yeasts were originally developed by subjecting yeasts to higher and higher alcohol levels during fermentation and selecting those cells that are still alive after a certain alcohol level had been reached....any dead yeast cells found will be ignored and discarded...
This process is repeated all the time brewing to higher levels of alcohol...until the yeast that live under the very highest levels are cropped and cultivated....these are then designated high alcohol tolerant.
It is an ongoing process to keep such yeast strains high alcohol tolerant and just because a certain strain of yeast says high alcohol tolerant does not mean it will stay that way over successive generations (brews)...taking yeast from the top of the very first krausen means that those yeast cells have never experienced high alcohol levels and so over a time they will eventually LOOSE their original inherited ability to withstand it!
The same situation is found can be found in bacteria and antibiotic resistance...only this is due to bacteria surviving after a small dose of anti-biotics is administered and rapidly developing genetic mutation resistance....
If you want to maintain the high alcohol tolerance of ale yeast then i would advise against top cropping!....you may be ok for a few brews but the yeast will eventually loose its alcohol resistance.....i suggest taking any yeast for cropping purposes AFTER fermentation is fully complete...any viable yeast still alive then even though stressed, will soon recover in fresh wort and still have its high alcohol tolerance attributes intact!
Yeast strains can be cultivated to adopt to various temps, alcohol tolerance and fermenting abilities its all very scientific nowadays....its always an ongoing battle to keep certain attributes intact...