Hi Paul How much are you brewing? a smack pack should have a population of 100 billion yeasts.
to use a smack pack remove it from the fridge the night before and let it warm to room temp slowly, and about 3-4 hours before you want to use it activate it by popping the inner bag.
clean and sanitise the pack before cutting and pouring out the contents when its swelled.
if older than optimum or u want a bigger population then you can make a starter, 100g of dme per litre will give u a liquor of 1040. if no dme sucrose will do. glass conical flasks u can sit on a gas flame are the best vessels for starters, easy to boil and sanitise with heat
tho pet bottles work for me too
to make the starter if you need one mix up your liquor and sanitise it sanitise the pet bottle if thats what your using and when cool enough add the yeast to the liquor cap with sanitised foil and give it a gentle swirl before sitting in a spot to incubate. give it a swirl when ever u walk past..
*** EDIT U canna sanatise pet with heat only chemically.
when the liquor is spent you can keep in the fridge till u need it.
when using pour off the spent liquor and discard before pitching in your beer.
you dont want to pitch the volume of spent liquor just the yeast