Yes - in the same way the boil will kill off any other microbes. But what it won't do is rid you of the lactic acid they produce. I use lactic acid sometimes as part of my normal water treatment but you need to be careful as it has quite a low taste threshold. I think that's the issue, along with whatever pleasant/unpleasant stuff any other microbes or enzymes present might start to produce.Will the boil kill Lactobacillus ?
Also bear in mind that one way of making a sour beer is kettle souring where you deliberately spike unhopped wort with lactobacillus - you leave it for a couple of days around 35degC and then boil it and hop it as normal.
I'm curious about this, though it must be said, not curious enough to actually waste my time trying it! Considering the time and temperatures involved in deliberately kettle souring I wonder how quickly you'd notice the effects of doing it accidentally like this, so to speak. And I suppose there's also the issue of whatever other microbes or enzymes might be present (I'm sure there's a vomit taste/aroma producing one...).you must make sure they do not drop below 50c as Lactobacillus can then start to present itself which will ruin your beer
I tried it a few times and also got significantly higher efficiency too (from 70 up to >80% kettle efficiency as I recall). The issue for me is I'm not as laid back and chilled out as @pilgrimhudd and get far too obsessed over numbers! I stopped doing it because I found it difficult to predict what the extra efficiency would be and so plan my brews accordingly. I do wonder though, now I use a mash re-circ pump whether I'd see much additional benefit from overnight mashing.Yes I do! Though only for 20l brews nowadays as like someone already said, it's a massive timesaver! I would also say that I do tend to get higher efficiencies by doing it. Bonus!