gurtpint said:
Is 5 days enough also when you step up from 1L to 2L starter?
There is no point in going from 1L to 2L. . . Best practice says step up by 10 times the volume so 1L to 10L.
When Working from slants I take a colony and inoculate 2ml of wort, when that is showing signs of
active fermentation, (usually 18-24 hours) I pitch that into 20ml of wort. When the 20ml is showing signs of
active fermentation, (usually another 18-24 hours), I pitch that into 200ml . . . When the 200ml is showing signs of
active fermentation, (usually another 36-48 hours). That gets pitched into 2L of wort . . . .Which ,for ales, I let work right out, decant off the spent wort and pitch the slurry. (Lagers get another step up only to 10L)
Letting the yeast work right out allows them to build up glycogen reserves so that they will be ready to go to work when pitched into fresh oxygenated wort.
If you are making a starter directly from a tube or a swollen smack pack then you only need to go straight to 5L there is no point doing anything smaller . . . unless you intend to split it out.
Chris Whites book has lots of stuff on starter size and why too many small steps is pointless.