When using modern malts is there any point in checking for conversion using iodine solution? Many brewers will say not, as most modern malts are so consistent they are âcertainâ to convert all of the starch to sugar. In the vast majority of cases they would be right, however, properly conducted, an iodine test tells a brewer so much more than âthe starch has been convertedâ.
At a pinch a dilute (12.5ppm) solution of iodophor can be used, but more consistent results can be obtained using a 0.02N solution of iodine (1.27g Iodine, 2.5g Potassium iodide in 500ml of distilled water). Place a sample of the mash liquid (ideally free of solids, as they will always turn blue black), in a white porcelain dish, then float several drops of iodine onto the extract, until a distinct layer is formed. Note any colour change in the iodine at its interface with the mash liquid. Also observe the intensity of the colour; is it trace, faint or strong?
The presence of starch is indicated by a blue-black colour and is fairly unmistakeable, gelatinised starch (amylose fragments, and large a-limit dextrins) produces a deep mahogany red brown colour. If the colour change is a faint red this indicates the presence of simple a-limit dextrins, a faint mahogany to violet reddish this shows the existence of small dextrins. Total mash saccharification (small a-limit dextrins with maltotriose, maltose and simple sugars) cause no change in the yellow colour of the iodine
Most of the starch should be reduced to at least small a-limit dextrins by alpha amylase. Even for sweet less fermented beers, the reaction should be no more than faintly mahogany to red. There should never be a strong colour reaction with the iodine; neither however, should rich beers be saccharified to the point that a negative iodine reaction occurs. A faint mahogany to reddish reaction indicates an acceptable extract composition for these beers.