Rapid thawing is thought to result in more viable yeast cells in a typical lab situation. Trying this with a 50mL vial, compared to a 1.5mL vial, though, might lead to localised heat shock and partial death? I donââ¬â¢t know, Iââ¬â¢m guessing. Youââ¬â¢d have to compare rapid vs slow...