Single step yeast starter

[DarrenSL]

Hi. Is there any advantage in stepping yeast starters up slowly rather than in one step so long as the cell count is the same?

I collected some (WLP830 Hefe iv) yeast slurry into sterile 10ml vials 2 weeks ago. I don't really have time to do a 2 step so was hoping to make a single 1.7L starter. Should give me c.250b cells which should be enough.

Thanks.

 

[Covrich]

I haven't got my book on me but I think you get a diminishing return after some point in the cell count growth

10ml isn't a lot because not all of it will be live yeast, you may stress the yeast a bit??

 

[Dutto]

One question ...

"How many times do you set off when your car is in third gear?"​

I rest my case! :thumb::thumb:

 

[Personal91]

One question ...

"How many times do you set off when your car is in third gear?"​

I rest my case! :thumb::thumb:

my mate managed to pull away in 5th gear before ! so it can be done

 

[strange-steve]

It's to do with inoculation rate rather than cell count. According to Chris White yeast has a maximum growth factor of 6 which is at an inoculation rate of about 5 billion cells per litre. A lower rate will not produce more yeast cells and so is pointless.
In other words if you put 5b yeast cells in a 2 litre starter you wouldn't get any more growth than if you'd put them in a 1 litre starter.

 

[Dutto]

my mate managed to pull away in 5th gear before ! so it can be done

Many years ago I occasionally found myself driving like that. :thumb: :thumb:

Too drunk to walk home so driving was the only option! :whistle: :whistle:

 

[Personal91]

Many years ago I occasionally found myself driving like that. :thumb: :thumb:

Too drunk to walk home so driving was the only option! :whistle: :whistle:

ahahaha :twisted::whistle: