Scientists Engineer Hopless Beer

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Scientists in California have developed a method enabling them to produce a beer without the use of hops, or the vast amounts of water it takes to grow them, raising questions on the environmentally-friendly future of beer production.

Researchers at UC Berkeley have instead found a way of engineering a strain of yeast that not only ferments the beer, but imparts the flavours traditionally provided by hops – the dried flowers of a climbing plant – eliminating the need for them in a brew.

Not only do hope require a lot of water, management, agricultural land, but the environmental impact involved in their transportation is huge, and are expensive, notes Charles Denby, one of two first authors of a paper appearing this week in the journal Nature Communication. Hops’ flavorful components also change year to year, much like wine grapes, and so standardized yeast would allow uniformity of flavor.

These impacts could be avoided by using yeast to make a hop-forward brew instead of cultivating hops.

“I started home brewing out of curiosity with a group of friends while I was starting out in Jay’s lab, in part because I enjoy beer and in part because I was interested in fermentation processes,” he said. “I found out that the molecules that give hops their hoppy flavor are terpene molecules, and it wouldn’t be too big of a stretch to think we could develop strains that make terpenes at the same concentrations that you get when you make beer and add hops to them.”

The engineered yeast strains were altered using a gene-editing tool invented at UC Berkeley, and were produced by inserting four new genes into industrial brewer’s yeast. This includes linalool synthase and geraniol synthase, which in this case were taken from mint and basil, and code for enzymes that produce flavor components. The two other genes were from yeast and boosted the production of precursor molecules needed to make linalool and geraniol, the hoppy flavor components.

In double-blind taste tests, employees of Lagunitas Brewing Company in Petaluma, California, said beer made from the engineered strains were “more hoppy” than a control beer made with regular yeast and Cascade hops.

“My hope is that if we can use the technology to make great beer that is produced with a more sustainable process, people will embrace that,” said Denby.

https://www.thedrinksbusiness.com/2018/03/scientists-engineer-environmentally-friendly-hopless-beer/

Interesting, but not sure I got into homebrew to use genetically modified yeasts!
 
The same could be said for growing barley or grapes for wine, might as well just drink water instead, nobody would mind.
 
I thought hops had an antiseptic/preservative effect and therefore beer without them would be far more susceptible to infection and not keep as long?
 
Read the paper yesterday, very interesting stuff. I met Jay Keasling a couple of times back when he was touring and lecturing about the genetically modified yeast he designed to produce the antimalarial drug artemisinin in large quantities at very low cost, cheap enough that it could be used by pretty much anyone in Africa or Latin America that needs it. Since then he has designed microbes to produce renewable alternatives to gasoline, diesel and jet fuel. I guess this hop project was just a side-show because he had the money and expertise to do it. He has a track record of creating spin-off companies so it might come to something, but I doubt it. GM yeast is a hard sell for those afraid of the unknown, even though brewers have been modifying yeast by selection for centuries. I know Chris White has ruled out stocking it at White Labs.

Incidentally, if anyone here wants to make their own GM yeast it is very simple and you can even buy kits with everything you need to insert GFP, a jellyfish protein that fluoresces green, into their genome along with all the necessary DNA to get it produced inside. They supply mead yeast, but you can sub it with any yeast you want to modify. Alternatively, you can sub out the GFP plasmid with one of your own. Simply send your DNA sequence of choice to a gene synthesis company (like this one), and bobs your uncle. I've been toying with some ideas about what sort of things could be inserted. Bacteriocins genes and their corresponding immunity proteins would be good candidates, as they could confer the ability to kill off bacteria and reduce the risk of getting the beer infected. Another alternative, which would have less chance of success, would be to insert enzymes from metabolic pathways which produce lactic acid, so that yeast could be used to make sour beers much as lactobacillus and pediococcus are.
 
I would lose interest if i could not use hops its part of the fun. No thanks Monsanto.

Maybe for some, part of the fun might be to engineer different flavoured yeasts. I think of this sort of thing a bit like the weird and whacky beers people brew - some people enjoy the unlimited creativity whereas others will stick with the traditional and consider a beer edgy if it uses a hop that originates from anywhere other than the South East. And then there are the many colours in between.
 
What would be great is instead of the hop flavours being part of the yeast, the hop oils were synthesised in a lab and we could add the essential oils and right amount of AAs to get the hop character we're after. We could pretty much 'build a beer' this way.
 
Not reading it yet but I'm wondering about bitterness, not just hop flavour. I can see a selection of yeasts being the base of the hop profile but unless you could mix the yeasts strains you'd still have to finesse with real hops.

Ideally we could 3d print the yeast from an app with hop sliders or those flavour spider diagrams.
 
Brewdog made a beer on there tv show that was made from distilled flavours made from tree bark and what not. Pitched no yeast. And juiced cucumbers instead of water
 
Brewdog made a beer on there tv show that was made from distilled flavours made from tree bark and what not. Pitched no yeast. And juiced cucumbers instead of water
Calling it beer is stretching it a bit.......
 
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