How many generations?

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In theory, if you are brewing regularly,(low gravity beers) or have really good yeast management, you can use the same yeast for ever.

I have used up to about 5 generations so far, then chucked it, (because i wasn't going to brew for a while.

I visited a commercial earlier this year that were on pitch 854.

It depends on a few things on how long you can use for.

1 Cleanliness
2 strength of wort
3 vessel your fermenting in
4 temp
5 nutrient levels#
6 when you skim/collect
7 what you do with it once you have skimmed

to name a few

Then there is always the mutation scenario, which sometimes can be benificial.

I wouldn't keep a dried yeast going for many generations, as those are the ones most likely to alter their charcteristics (mutate)

UP
 
I've been reusing a portion of the slurry mainly. Trying my best to collect the best bit.

did a nice technique yesterday though....

Brewed a beer thursday night and pitched about 100ml of 3rd generation wlp051 that was looking a bit dirty to be honest. The next day when bubbles started coming out the airlock at about 1 per minute (before a head formed, just a few bubbles), I dropped the lot into a fresh FV, leaving behind the dead yeast and **** that was in with the yeast slurry.

I wouldnt normally do this as I usually get a great start on fermentation after a few hours but on this occasion the drop happened after about 18 hours.
 

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