Brewlabs Sussex 1

[ManseMasher]

Just got my Brewlabs Sussex 1 yeast through the post (with a view to brewing a Harvey's Best clone). Anyone used this before? It's on an agar slope - do I wash it with wort? As in dunk it in and swirl it around, or pour wort over it into the jar I use to generate my starters?

 

[clibit]

"To grow the yeast from the slope prepare a nutrient solution of malt broth by dissolving three table spoons of malt extract in 300 cm³ of boiled water in a clean sterilised flask or bottle of at least 500 cm³ size.
When cool (below 35 C) pour some of the solution onto the yeast culture and shake to suspend the yeast cells. Alternatively a flamed wire loop may be used to loosen the cells. DO NOT ATTEMPT TO DISSOLVE THE SLOPE. THE CELLS ONLY GROW ON THE SURFACE OF THE AGAR AND SHOULD BE DISLODGED BY SHAKING.
Transfer the yeast suspension into the rest of the nutrient solution and allow to grow for 24 to 48 hours in a warm environment (20-30 C) as a starter culture in your brew. Cover the bottle opening with aluminium foil or plug with cotton wool. DO NOT INCUBATE A STARTER CULTURE IN A TIGHTLY SEALED BOTTLE AS PRESSURE MAY CAUSE IT TO EXPLODE.
Ensure that the yeast is working well before pitching. It should show a frothy surface as gas is released and have a good sediment of yeast cells at the bottom. Ensure that all of these are pitched into your wort to achieve maximum speed of fermentation.
Take care when handling the culture and starter bottles to avoid contamination particularly from fingers."

 

[ManseMasher]

That'll do nicely sir. Ever used it?

 

[clibit]

No. But I have a Whitelabs vial in the fridge which my mate gave me, and on the side in marker pen it says 'Sussex'. So it could well be from the Brewlabs strain, he didn't say.