I do a lot of yeast ranching with these slants. My process is take sterile loop full and innoculate into 15 ml of sterile 1.040 wort, grow for 2 days warm then add to 100 ml sterile 1.040 wort, all made with 1g DME per 10 ml. Then to get final starter for 5 or 6 gallon batch add 100 ml of fully...